CST, 5091S, HIPK2 Antibody

Polyclonal Antibody for studying HIPK2. Validated for Western Blotting. Highly specific and rigorously validated in-house, HIPK2 Antibody (CST #5091) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity HIPK2 Antibody detects endogenous levels of total HIPK2 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln1045 of human HIPK2 protein. Antibodies were purified by protein A and peptide affinity chromatography. Background Members of the homeodomain-interacting protein kinase (HIPK1-4) family of serine/threonine kinases regulate gene transcription with effects on cell proliferation, differentiation, and apoptosis (1-3). HIPK1-3 are nuclear proteins that were originally described as co-repressors for homeobox transcription factors (1). HIPK proteins can interact with and/or phosphorylate many transcriptional regulators (4). HIPK2 activated in response to DNA damage, including UV radiation and chemotherapeutic drugs, phosphorylates p53 at Ser46 to promote the transcription of pro-apoptotic p53 target genes (5-7). In addition, HIPK2 interacts with a number of transcription factors that control developmental processes, tumor suppression and apoptosis (4). The kinase is regulated by both sumoylation (8) and ubiquitination (9,10). Ubiquitination and subsequent degradation of HIPK2 is inhibited by DNA damaging agents. Caspase-dependent cleavage of HIPK2 removes the inhibitory domain and results in enhanced HIPK2 activity (11). Alternate Names DKFZp686K02111; FLJ23711; hHIPk2; HIPK2; homeodomain interacting protein kinase 2; Homeodomain-interacting protein kinase 2; PRO0593 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 130-140 SOURCE: Rabbit