CST, 50945S, DUSP6/MKP3 Antibody

Polyclonal Antibody for studying DUSP6/MKP3. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, DUSP6/MKP3 Antibody (CST #50945) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity DUSP6/MKP3 Antibody recognizes endogenous levels of total DUSP6/MKP3 protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val372 of human DUSP6/MKP3 protein. Antibodies are purified peptide affinity chromatography. Background MAP kinases are inactivated by dual-specificity protein phosphatases (DUSPs) that differ in their substrate specificity, tissue distribution, inducibility by extracellular stimuli, and cellular localization. DUSPs, also known as MAPK phosphatases (MKPs), specifically dephosphorylate both threonine and tyrosine residues in MAPK P-loops and have been shown to play important roles in regulating the function of the MAPK family (1,2). At least 13 members of the family (DUSP1-10, DUSP14, DUSP16, and DUSP22) display unique substrate specificities for various MAP kinases (3). MAPK phosphatases typically contain an amino-terminal rhodanese-fold responsible for DUSP docking to MAPK family members and a carboxy-terminal catalytic domain (4). These phosphatases can play important roles in development, immune system function, stress responses, and metabolic homeostasis (5). In addition, research studies have implicated DUSPs in the development of cancer and the response of cancer cells to chemotherapy (6). Alternate Names dual specificity phosphatase 6; Dual specificity protein phosphatase 6; Dual specificity protein phosphatase PYST1; DUS6; DUSP6; HH19; MAP kinase phosphatase 3; Mitogen-activated protein kinase phosphatase 3; MKP-3; MKP3; PYST1; serine/threonine specific protein phosphatase Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 42 SOURCE: Rabbit