CST, 51030SF, Phospho-Rad50 (Ser635) (F5H8B) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying Rad50 (Ser635) phosphate. Validated for Western Blotting,Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, Phospho-Rad50 (Ser635) (F5H8B) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #51030) is ready to ship. Product Usage Information This product is the carrier free version of product #99215. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 99215 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Phospho-Rad50 (Ser635) (F5H8B) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of Rad50 protein only when phosphorylated at Ser635. Species Reactivity: Human, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser635 of human Rad50 protein. Background The DNA repair protein Rad50 is a member of the structural maintenance of chromosomes (SMC) family and plays an important role in cell cycle checkpoint signaling and double-strand break repair in response to DNA damage (1-4). Rad50 forms a complex with Mre11 and NBS1 that becomes activated in response to DNA damage (3). In normal human cells, the MRN complex acts to tether linear DNA molecules, providing a flexible link between DNA ends (1). Genomic instability and cancer have been shown to develop in cells with genetic mutations affecting the proteins in the MRN complex (2). ATM-dependent phosphorylation of Rad50 at Ser635 in response to DNA damage is important in regulating downstream signaling, DNA repair, and checkpoint control (5). Alternate Names DNA repair protein RAD50; hRAD50; NBSLD; RAD50; RAD50 double strand break repair protein; RAD50 homolog (S. cerevisiae); RAD50 homolog, double strand break repair protein; RAD50-2; RAD502 Specification REACTIVITY: H R SENSITIVITY: Endogenous MW (kDa): 153 Source/Isotype: Rabbit IgG