CST, 51076T, NSD1 (E2U6H) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying NSD1. Validated for Western Blotting,Chromatin IP. Available in 2 sizes. Highly specific and rigorously validated in-house, NSD1 (E2U6H) Rabbit Monoclonal Antibody (CST #51076) is ready to ship. Product Usage Information For optimal ChIP results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 × 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Chromatin IP: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Chromatin IP Specificity / Sensitivity NSD1 (E2U6H) Rabbit Monoclonal Antibody recognizes endogenous levels of total NSD1 protein. This antibody recognizes both the NSD1 long isoform (UniProt #Q96L73-1) and the short isoform (UniProt #Q96L73-2). Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human NSD1 protein. Background Nuclear receptor SET domain-containing protein 1 (NSD1) is a member of the NSD family of histone methyltransferases, including WHSC1/NSD2 and WHSC1L1/NSD3. NSD1 specifically catalyzes mono- and di-methylation of histone H3 at Lys36 (H3K36me), a mark that is typically associated with transcriptionally active regions of the genome (1,2). NSD1 is expressed as two isoforms: a predominantly expressed short isoform of roughly 2,400 amino acids, and a less abundant long isoform of nearly 2,700 amino acids (3). NSD1 is required for proper embryonic development, as deletion of NSD1 is embryonic lethal in mice (4). Mutations in NSD1 are also believed to be the major cause of Sotos syndrome, an overgrowth disorder characterized by pre- and post-natal overgrowth, macrocephaly, advanced bone age, and developmental delay (5-7). NSD1 mutations have additionally been documented in two related overgrowth disorders, Weaver syndrome and Beckwith-Wiedemann syndrome, albeit less frequently (6-8). NSD1 is also mutated or has altered expression in several types of cancer. For instance, NSD1 is upregulated in metastatic prostate cancer and hepatocellular carcinoma (HCC) compared to healthy tissue (9,10). Loss-of-function mutations have also been observed for NSD1 in head and neck squamous cell carcinomas (HNSCCs), and the gene is epigenetically silenced in neuroblastoma and glioma cells (11,12). NSD1, therefore, appears to exert tumor suppressive or promoting functions depending on cellular context, either through promoting the expression of tumor suppressor or oncogenic proteins, respectively (13). Alternate Names Androgen receptor coactivator 267 kDa protein; androgen receptor-associated coregulator 267; Androgen receptor-associated protein of 267 kDa; ARA267; DKFZp666C163; FLJ10684; FLJ22263; FLJ44628; H3-K36-HMTase; H4-K20-HMTase; Histone-lysine N-methyltransferase, H3 lysine-36 and H4 lysine-20 specific; Histone-lysine N-methyltransferase, H3 lysine-36 specific; KMT3B; Lysine N-methyltransferase 3B; NR-binding SET domain-containing protein; NSD1; nuclear receptor binding SET domain protein 1; nuclear receptor SET domain-containing protein 1; Nuclear receptor-binding SET domain-containing protein 1; SOTOS; SOTOS1; STO; truncated nuclear receptor binding SET domain protein 1 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 300 Source/Isotype: Rabbit IgG