CST, 51077SF, ATP-Citrate Lyase (D1X6P) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying ATP Citrate Lyase. Validated for Western Blotting. Highly specific and rigorously validated in-house, ATP-Citrate Lyase (D1X6P) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #51077) is ready to ship. Product Usage Information This product is the carrier free version of product #13390. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 13390 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity ATP-Citrate Lyase (D1X6P) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total ATP-citrate lyase protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val34 of human ATP citrate lyase protein. Background ATP-citrate lyase (ACL) is a homotetramer that catalyzes the formation of acetyl-CoA and oxaloacetate (OAA) in the cytosol, which is the key step for the biosynthesis of fatty acids, cholesterol, and acetylcholine, as well as for gluconeogenesis (1). Nutrients and hormones regulate the expression level and phosphorylation of ATP-citrate lyase (1,2). It is phosphorylated by GSK-3 on Thr446 and Ser450 (3). Ser455 of ATP-citrate lyase has been reported to be phosphorylated by PKA and Akt (4,5). Phosphorylation on Ser455 abolishes the homotropic allosteric regulation by citrate and enhances the catalytic activity of the enzyme (2). Alternate Names ACL; ACLY; ATP citrate lyase; ATP-citrate (pro-S-)-lyase; ATP-citrate synthase; ATPCL; Citrate cleavage enzyme; CLATP Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 125 Source/Isotype: Rabbit IgG