CST, 51124T, TPH2 (D3E5I) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TPH2. Validated for Western Blotting,Immunofluorescence (Frozen). Available in 2 sizes. Highly specific and rigorously validated in-house, TPH2 (D3E5I) Rabbit Monoclonal Antibody (CST #51124) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Frozen): 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Frozen) Specificity / Sensitivity TPH2 (D3E5I) Rabbit Monoclonal Antibody recognizes endogenous levels of total TPH2 protein. Species cross-reactivity for Western blot is human and rat only. Mouse cross-reactivity is determine by immunofluoresence. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu30 of human TPH2 protein. Background Tryptophan hydroxylase (TPH) is the rate-limiting enzyme in the biosynthesis of serotonin (1) by converting tryptophan to 5-hydroxy-L-tryptophan (2). Two isoforms of TPH exist: TPH-1 is mainly expressed in the periphery, whereas the expression of TPH-2 is restricted to neuronal cells and the central nervous system (3). Most of the serotonin found throughout the body is synthesized by TPH-1 in enterochromaffin cells of the gastrointestinal tract. Targeted disruption of the gene results in low levels of circulating and tissue serotonin (4). Polymorphisms and regulation of the gene are involved in cognitive, emotional, and psychiatric disorders (5-7) Alternate Names ADHD7; FLJ37295; MGC138871; MGC138872; Neuronal tryptophan hydroxylase; NTPH; TPH2; Tryptophan 5-hydroxylase 2; Tryptophan 5-monooxygenase 2; tryptophan hydroxylase 2 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 52 Source/Isotype: Rabbit IgG