CST, 51142S, Angiomotin (E3E7X) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying AMOT. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Angiomotin (E3E7X) Rabbit Monoclonal Antibody (CST #51142) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Angiomotin (E3E7X) Rabbit Monoclonal Antibody recognizes endogenous levels of angiomotin isoform 1 (full-length) protein. This antibody does not recognize the amino-terminally truncated angiomotin isoform 2 protein. This antibody cross-reacts with the angiomotin family members, angiomotin-like 1 (AMOTL1) and angiomotin-like 2 (AMOTL2). Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human angiomotin protein. Background Angiomotin (AMOT) is a widely expressed cell junction protein initially identified through its ability to bind angiostatin. Alternative splicing results in two isoforms of angiomotin, the full-length p130 and the amino-terminally truncated p80. These isoforms, along with angiomotin-like 1 (AMOTL1) and angiomotin-like 2 (AMOTL2), comprise the Motin protein family. Angiomotin is important in endothelial cell polarity, migration, and blood vessel formation during development, as well as in signaling through small GTPases and the Hippo/YAP pathway (reviewed in 1). Research studies have shown that angiomotin expression regulates migration and proliferation of breast cancer cells (2,3). Alternate Names AMOT; Angiomotin; angiomotin p130 isoform; angiomotin p80 isoform; KIAA1071 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 140, 130, 100 Source/Isotype: Rabbit IgG