CST, 51201SF, WRN (8H3) Mouse Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying WRN. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, WRN (8H3) Mouse Monoclonal Antibody (BSA and Azide Free) (CST #51201) is ready to ship. Product Usage Information This product is the carrier free version of product #4666. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 4666 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity WRN (8H3) Mouse Monoclonal Antibody (BSA and Azide Free) detects endogenous levels of total WRN protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with full length human recombinant WRN protein. Background The RecQ family of DNA and RNA helicases is a family of enzymes that has been shown to be important to genome integrity (1). Members of this family function in several DNA repair processes, including double-strand break repair, homologous recombination, and re-initiation of DNA replication at stalled replication forks (2,3). Mutations in RecQ helicase family members result in syndromes that display varying types of chromosomal abnormalities and overall genomic instability (1). is a member of the RecQ family that has been identified as the gene underlying Werner's syndrome, an autosomal recessive disorder characterized by premature aging and predisposition to cancer (4). Cells from Werner's syndrome patients exhibit genomic instability that is associated with deficient DNA repair activity (5). Alternate Names Bifunctional 3'-5' exonuclease/ATP-dependent helicase WRN; DKFZp686C2056; DNA helicase, RecQ-like type 3; Exonuclease WRN; RecQ protein-like 2; RECQ3; RECQL2; RECQL3; Werner syndrome ATP-dependent helicase; Werner syndrome RecQ like helicase; Werner syndrome, RecQ helicase-like; WRN; WRN RecQ like helicase Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 200 Source/Isotype: Mouse IgG1