CST, 5122S, FAM129B Antibody

Polyclonal Antibody for studying FAM129B. Validated for WB,IP,IF,F. Highly specific and rigorously validated in-house, FAM129B Antibody (CST #5122) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:50 Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity FAM129B Antibody recognizes endogenous levels of total FAM129B protein. Species Reactivity: Human, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu705 of human Fam129B protein. Antibodies were purified by protein A and peptide affinity chromatography. Background FAM129B/Niban-like protein 1 (family with sequence similarity 129, member B) belongs to a poorly characterized family of Niban proteins that also includes FAM129A/Niban and FAM129C/Niban-like protein 2. FAM129A/Niban is implicated in the ER stress response and is upregulated at the protein level in thyroid carcinoma (1,2). FAM129C/Niban-like protein 2 is preferentially expressed in B-cells and is one of five biomarkers upregulated in whole blood from patients with colorectal carcinoma (3,4). FAM129B is broadly expressed and has been shown to be a downstream target of B-Raf in melanoma cells (5). Though FAM129B does not appear to regulate cell growth and division, phosphorylation of FAM129B by B-Raf is essential for the invasive potential of melanoma and non-melanoma cell lines (5). Deletion of FAM129B in melanoma cells significantly impairs B-Raf/MEK/Erk-dependent invasion into the extracellular matrix (5). Alternate Names bA356B19.6; C9orf88; DKFZP434H0820; FAM129B; family with sequence similarity 129 member B; family with sequence similarity 129, member B; FLJ13518; FLJ22151; FLJ22298; Meg-3; Melanoma invasion by ERK; MINERVA; NIBA2; niban apoptosis regulator 2; Niban-like protein 1; NIBAN2; NIBL1; OC58; Protein FAM129B; Protein Niban 2 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 95 SOURCE: Rabbit