CST, 51258S, CUL4A (F5A5N) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CUL4A. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, CUL4A (F5A5N) Rabbit Monoclonal Antibody (CST #51258) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity CUL4A (F5A5N) Rabbit Monoclonal Antibody recognizes endogenous levels of total CUL4A protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human CUL4A protein. Background Cullin-4 (CUL4) is a member of the cullin family of related ubiquitin ligases (1). A pair of mammalian CUL4 proteins have been identified: CUL4A and CUL4B. The carboxy-terminal CUL4 domain interacts with Rbx1 and E2 enzymes, while the amino-terminal CUL4 domain interacts with the BPB domain of UV-damaged DNA-binding protein DDB1 to form a CUL4-DDB1 ubiquitin ligase complex (2). This CUL4-DDB1 complex binds a variety of WD40-containing proteins that help to enhance additional substrate recruitment (2,3). The CUL4 complex has been shown to target substrates involved in DNA repair, cell cycle progression (2), transcription (3), and development (2-6). Alternate Names CUL-4A; CUL4A; cullin 4A; Cullin-4A Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 80, 82 Source/Isotype: Rabbit IgG