CST, 5137S, TIAR Antibody

Polyclonal Antibody for studying TIAR. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, TIAR Antibody (CST #5137) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity TIAR Antibody detects endogenous levels of total TIAR protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro337 of human TIAR. Antibodies are purified by protein A and peptide affinity chromatography. Background TIAR is a member of the RNA-recognition motif (RRM) family of RNA-binding proteins (1,2). It functions as a translational repressor under conditions of cellular damage (3,4). In response to cellular stress, TIAR associates with eIF1, eIF3, and the 40S ribosomal subunit and forms noncanonical preinitiation complexes that are translationally inactive (3,4). TIAR then aggregates with its family member TIA1 and facilitates the accumulation of the translationally inactive preinitiation complexes into discrete cytoplasmic foci called stress granules. The two major isoforms of TIAR are the products of alternative mRNA splicing (5,6). Alternate Names aging-associated gene 7 protein; MGC33401; Nucleolysin TIAR; T-cluster binding protein; TCBP; TIA-1 related protein; TIA-1-related nucleolysin; TIA-1-related protein; TIA1 cytotoxic granule associated RNA binding protein like 1; TIA1 cytotoxic granule-associated RNA binding protein-like 1; TIA1 cytotoxic granule-associated RNA-binding protein-like 1; TIA1 related; TIAL1; TIAR Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 42 SOURCE: Rabbit