CST, 51398S, Elk-1 (F6D8T) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Elk-1. Validated for Western Blotting,Immunoprecipitation,Chromatin IP. Available in 2 sizes. Highly specific and rigorously validated in-house, Elk-1 (F6D8T) Rabbit Monoclonal Antibody (CST #51398) is ready to ship. Product Usage Information For optimal ChIP results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 × 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Immunoprecipitation: 1:200 Chromatin IP: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Chromatin IP Specificity / Sensitivity Elk-1 (F6D8T) Rabbit Monoclonal Antibody recognizes endogenous levels of total Elk-1 protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro423 of human Elk-1 protein. Background The ETS transcription factor Elk-1 is a component of the ternary complex that binds the serum response element (SRE) and mediates gene activity in response to serum and growth factors (1-3). Elk-1 is phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its carboxy terminus; phosphorylation at these sites, particularly Ser383, is critical for transcriptional activation by Elk-1. Elk-1 appears to be a direct target of activated MAP kinase: (a) biochemical studies indicate that Elk-1 is a good substrate for MAP kinase; (b) the kinetics of Elk-1 phosphorylation and activation correlate with MAP kinase activity; (c) interfering mutants of MAP kinase block Elk-1 activation . Other studies have shown that Elk-1 (Ser383) is also a target of the stress-activated kinase SAPK/JNK (4,5). Alternate Names Elk-1; ELK1; ELK1, ETS transcription factor; ELK1, member of ETS oncogene family; ETS domain-containing protein Elk-1; ETS transcription factor ELK1; ETS-like gene 1; tyrosine kinase (ELK1) oncogene Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 65 Source/Isotype: Rabbit IgG