CST, 51418T, Cyclophilin A (D2Y4M) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PPIA. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Cyclophilin A (D2Y4M) Rabbit Monoclonal Antibody (CST #51418) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10mM sodium HEPES (pH 7.5), 150mM NaCl, 100 μg/ml, 50% glycerol and less than 0.02% sodium azide. Store at -20ºC. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Cyclophilin A (D2Y4M) Rabbit Monoclonal Antibody recognizes endogenous levels of total cyclophilin A protein. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly18 of mouse cyclophilin A protein. Background Cyclophilins are a highly conserved family of peptidylprolyl - -isomerases (PPIA) that are targets of the immunosuppressant drug cyclosporin A (CsA) (1,2). The complex of cyclophilin and CsA can bind to and inhibit calcineurin which leads to inhibition of the transcription factor NFAT and decreased production of cytokines (3,4). As isomerases, cyclophilins have been proposed to aid in protein folding. Cyclophilin A can bind to the p55 Gag protein of HIV and appears necessary for HIV infection (5,6). There is also some evidence that cyclophilins have nuclease activity and play a role in apoptosis (7). Alternate Names Cyclophilin A; Cyclosporin A-binding protein; CYPA; CYPH; epididymis secretory sperm binding protein Li 69p; HEL-S-69p; MGC117158; MGC12404; MGC23397; Peptidyl-prolyl cis-trans isomerase A; Peptidyl-prolyl cis-trans isomerase A, N-terminally processed; peptidylprolyl isomerase A; peptidylprolyl isomerase A (cyclophilin A); PPIA; PPIase A; Rotamase A; T cell cyclophilin Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 18 Source/Isotype: Rabbit IgG