CST, 5146T, HGK (D19H10) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying HGK. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, HGK (D19H10) Rabbit Monoclonal Antibody (CST #5146) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity HGK (D19H10) Rabbit Monoclonal Antibody detects endogenous levels of total HGK protein. Species Reactivity: Human, Mouse, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala553 of human HGK protein. Background HGK (MAP4K4 or mitogen-activated protein kinase kinase kinase kinase 4) is a serine/threonine kinase that belongs to the mammalian STE20/MAP4K kinase family involved in response to environmental stress and cytokines such as TNF-α (1-3). HGK specifically activates the c-Jun N-terminal kinase (JNK) signaling pathway and increases AP-1-mediated transcriptional activity (1). HGK is broadly expressed in many types of human cancer and cancer cell lines and plays an important role in cell transformation, invasiveness, adhesion and migration (4,5). Alternate Names epididymis secretory protein Li 31; FLH21957; FLJ10410; FLJ20373; FLJ90111; HEL-S-31; hepatocyte progenitor kinase-like/germinal center kinase-like kinase; HGK; HPK/GCK-like kinase HGK; KIAA0687; M4K4; MAP4K4; MAPK/ERK kinase kinase kinase 4; MEK kinase kinase 4; MEKKK 4; MEKKK4; Mitogen-activated protein kinase kinase kinase kinase 4; Nck-interacting kinase; NIK; Ste20 group protein kinase HGK; ZC1 Specification REACTIVITY: H M Mk SENSITIVITY: Endogenous MW (kDa): 150 Source/Isotype: Rabbit IgG