CST, 5147S, SRF (D71A9) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SRF. Validated for Western Blotting,Immunoprecipitation,Chromatin IP. Available in 2 sizes. Highly specific and rigorously validated in-house, SRF (D71A9) Rabbit Monoclonal Antibody (CST #5147) is ready to ship. Product Usage Information For optimal ChIP results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Immunoprecipitation: 1:50 Chromatin IP: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Chromatin IP Specificity / Sensitivity SRF (D71A9) Rabbit Monoclonal Antibody recognizes endogenous levels of total SRF protein. Species Reactivity: Human, Mouse, Rat, Monkey, Pig Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser375 of human SRF protein. Background The serum response factor (SRF) is a 67 kDa phospho-protein that, together with auxiliary factors, modulates transcription of immediate early genes containing serum response elements at their promoters (1,2). SRF contains several phosphorylation sites (3), but functional consequences of phosphorylation have not been identified unequivocally. Several growth factor- and calcium-regulated kinases, such as p90RSK and CaM kinase IV, can phosphorylate SRF at Ser103 (4,5), and Ser103 of SRF is also a nuclear target for MAPKAP kinase 2 (6). Alternate Names c-fos serum response element-binding transcription factor; MCM1; minichromosome maintenance 1 homolog; Serum response factor; serum response factor (c-fos serum response element-binding transcription factor); SRF Specification REACTIVITY: H M R Mk Pg SENSITIVITY: Endogenous MW (kDa): 67 Source/Isotype: Rabbit IgG