CST, 51509T, GAK (E7W7Z) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying GAK. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, GAK (E7W7Z) Rabbit Monoclonal Antibody (CST #51509) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity GAK (E7W7Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total GAK protein. A band of unknown origin is detected at ~45 kDa and at ~20kDa. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro758 of human GAK protein. Background Cyclin-G-associated kinase (GAK) is the ubiquitous form of the neuronal-specific protein auxilin 1 (1) and is an essential cofactor for Hsc70-dependent uncoating of clathrin-coated vesicles in non-neuronal cells (2). The recruitment of GAK to clathrin-coated pits during endocytosis is dependent on its PTEN-like domain, which has been shown to bind to phospholipids (3). It has been postulated that the JAK inhibitor baricitinib may impair entry of the SARS-CoV-2 virus by inhibiting AAK1- and GAK-mediated endocytosis via ACE2 (4). In the nucleus, GAK forms complexes with cyclin G1 and other nuclear proteins (5) and plays an essential role in proper mitotic progression by maintaining centrosome structure (6). GAK is also required for the regulation of basal, PRKN-independent mitophagy by modifying the mitochondrial network and lysosomal morphology that compromise efficient transport of mitochondria for degradation (7,8). Alternate Names auxilin-2; cyclin G associated kinase; Cyclin-G-associated kinase; DNAJ26; DNAJC26; FLJ16629; FLJ40395; GAK; MGC99654 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 175 Source/Isotype: Rabbit IgG