CST, 51593T, ZAP (E7E8D) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ZAP. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, ZAP (E7E8D) Rabbit Monoclonal Antibody (CST #51593) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity ZAP (E7E8D) Rabbit Monoclonal Antibody recognizes endogenous levels of total ZAP protein. This antibody recognizes ZAP isoform 1. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp699 of human ZAP protein. Background ZAP (Zinc finger CCCH-type antiviral protein 1, ZC3HAV1, PARP13) is an RNA-binding protein which inhibits the replication of certain viruses, including HIV-1, by preventing the accumulation of viral RNA in the cytoplasm (1-5). ZAP binds to specific viral mRNAs through the CCCH zinc finger motifs located on its N terminus, and recruits exosomes and decapping enzymes to degrade the target RNA (3-5). ZAP also interferes with the interaction between eIF4A and eIF4G on target mRNA, causing translational repression (6). Isoform 2, the shorter isoform also known as ZAPS, acts as a stimulator of interferon responses in human cells mediated by the RNA helicase RIG-I as part of the innate antiviral immune response (7). Alternate Names ADP-ribosyltransferase diphtheria toxin-like 13; ARTD13; CCCH-type zinc finger antiviral protein; DKFZp686F2052; DKFZp686H1869; DKFZp686O19171; FLB6421; FLJ13288; Inactive Poly [ADP-ribose] polymerase 13; MGC48898; PARP13; ZAP; ZC3H2; ZC3HAV1; ZC3HDC2; ZCCHV; Zinc finger antiviral protein; Zinc finger CCCH domain-containing protein 2; zinc finger CCCH type, antiviral 1; Zinc finger CCCH-type antiviral protein 1; zinc finger CCCH-type containing, antiviral 1; zinc finger CCCH-type, antiviral 1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 100 Source/Isotype: Rabbit IgG