CST, 51623T, Phospho-Syntenin-1/MDA9 (Ser60) (F4T2K) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying syntenin (Ser60) phosphate. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Syntenin-1/MDA9 (Ser60) (F4T2K) Rabbit Monoclonal Antibody (CST #51623) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Phospho-Syntenin-1/MDA9 (Ser60) (F4T2K) Rabbit Monoclonal Antibody recognizes transfected levels of syntenin-1/MDA9 protein only when phosphorylated at Ser60. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser60 of human syntenin-1/MDA9 protein. Background Syntenin-1, also known as MDA9 or SDCBP, is a member of the PDZ family of proteins (1), defined by the presence of a PDZ protein interaction domain. Syntenin-1, via its PDZ domain, functions as a scaffold adaptor protein that regulates numerous signal transduction pathways, including FAK, Src, TGF-βR1, EGFR, and IGF1R (2-5). Aberrantly elevated expression of syntenin-1 has been found in various cancers and has been linked to the growth and invasion of tumor cells (5-7). In the central nervous system, syntenin-1 has been shown to mediate neuron dendrite protrusion and spine formation through its interaction with syndecan and Rheb (8). Syntenin-1 is also a key component of the syndecan/syntenin/ALIX pathway, and it has been shown to contribute directly to the formation and internalization of exosomes (9-11). Syntenin-1/MDA9 has been shown to be phosphorylated at Ser61 by ULK1 in mouse, which is highly conserved with Ser60 in human (12). Alternate Names MDA-9; MDA9; melanoma differentiation associated protein-9; Melanoma differentiation-associated protein 9; Pro-TGF-alpha cytoplasmic domain-interacting protein 18; Scaffold protein Pbp1; SDCB1; SDCBP; ST1; SYCL; syndecan binding protein; syndecan binding protein (syntenin); Syndecan-binding protein 1; syntenin; Syntenin-1; TACIP18 Specification REACTIVITY: H SENSITIVITY: Transfected Only MW (kDa): 30 Source/Isotype: Rabbit IgG