CST, 51634S, C-Peptide (E9C4P) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying insulin C-peptide. Validated for Immunohistochemistry (Paraffin),Immunofluorescence (Frozen). Available in 2 sizes. Highly specific and rigorously validated in-house, C-Peptide (E9C4P) Rabbit Monoclonal Antibody (CST #51634) is ready to ship. Product Usage Information Immunohistochemistry (Paraffin): 1:500 - 1:2000 Immunofluorescence (Frozen): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Immunohistochemistry (Paraffin), Immunofluorescence (Frozen) Specificity / Sensitivity C-Peptide (E9C4P) Rabbit Monoclonal Antibody recognizes endogenous levels of total C-peptide protein. Species Reactivity: Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surroundind Gly71 of human C-peptide protein. Background Glucose homeostasis is regulated by hormones. Elevation of blood glucose levels during feeding stimulates insulin release from pancreatic β-cells through a glucose sensing pathway (1). Proinsulin, the insulin precursor molecule, is processed prior to its secretion. Insulin is composed of A-peptide and B-peptide which are joined by a disulfide bond. The center one-third of the molecule is cleaved and released as C-peptide, which has a longer half-life than insulin (2). Alternate Names IDDM; IDDM1; IDDM2; ILPR; ins; Insulin; insulin (A chain, B chain); Insulin A chain; Insulin B chain; IRDN; MODY10; PNDM4; preproinsulin; proinsulin Specification REACTIVITY: M R SENSITIVITY: Endogenous MW (kDa): 4 Source/Isotype: Rabbit IgG