CST, 51658S, beta2A-Tubulin Antibody

Polyclonal Antibody for studying TUBB2A. Validated for Western Blotting. Highly specific and rigorously validated in-house, beta2A-Tubulin Antibody (CST #51658) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity beta2A-Tubulin Antibody recognizes endogenous levels of total β2A-tubulin protein. This antibody does not cross-react with β2B-tubulin protein. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser201 of human β2A-tubulin protein. Antibodies are purified by peptide affinity chromatography. Background The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1). Tubulinopathies cover a spectrum of disorders affecting neuronal development. Mutations in β-tubulin genes, including the β2A-tubulin gene , are associated with cortical and extracortical malformations, leading to cognitive deficits and epilepsy (2). β2A-tubulin has also been associated with the formation of tau inclusions, and is a suggested biomarker for breast cancer metastasis (3,4). Alternate Names CDCBM5; class IIa beta-tubulin; dJ40E16.7; TBB2A; TUBB; TUBB2; TUBB2A; tubulin beta 2A class IIa; Tubulin beta class IIa; Tubulin beta-2A chain; tubulin, beta 2A; tubulin, beta polypeptide 2 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 55 SOURCE: Rabbit