CST, 51676T, AIFM2/FSP1 (F7A3L) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying AIFM2/FSP1. Validated for Western Blotting,Simple Western™,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, AIFM2/FSP1 (F7A3L) Rabbit Monoclonal Antibody (CST #51676) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Immunofluorescence (Immunocytochemistry): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity AIFM2/FSP1 (F7A3L) Rabbit Monoclonal Antibody recognizes endogenous levels of total AIFM2/FSP1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human AIFM2/FSP1 protein. Background Apoptosis-inducing factor mitochondria-associated 2 (AIFM2), also known as AIF-homologous mitochondrion-associated inducer of death (AMID) or p53-responsive gene 3 (PRG3), is a homolog of apoptosis-inducing factor (AIF) (1,2). Research studies show that it inhibits ferroptosis, a form of cell death caused by iron-dependent lipid peroxidation. Therefore, this protein was named as ferroptosis suppressor protein 1 (FSP1). FSP1 is a coenzyme Q (CoQ) oxidoreductase and generates the reduced form of (CoQ), which traps lipid peroxides, leading to the suppression of ferroptosis. Furthermore, inhibition of ferroptosis by FSP1 was shown to be independent of GPX4, another negative regulator of ferroptosis (3,4). Alternate Names 5430437E11Rik; AIFM2; AMID; apoptosis inducing factor mitochondria associated 2; apoptosis-inducing factor (AIF)-homologous mitochondrion-associated inducer of death; apoptosis-inducing factor (AIF)-like mitochondrion-associated inducer of death; Apoptosis-inducing factor 2; Apoptosis-inducing factor homologous mitochondrion-associated inducer of death; Apoptosis-inducing factor-like mitochondrion-associated inducer of death; apoptosis-inducing factor, mitochondrion-associated, 2; Ferroptosis suppressor protein 1; FSP1; p53-responsive gene 3 protein; PRG3; RP11-367H5.2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 41 Source/Isotype: Rabbit IgG