CST, 5169T, eIF4G2/p97 (D88B6) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying eIF4G2/DAP5. Validated for WB,IP,IF,F,eCLIP. Available in 2 sizes. Highly specific and rigorously validated in-house, eIF4G2/p97 (D88B6) Rabbit Monoclonal Antibody (CST #5169) is ready to ship. Product Usage Information For more information about the RBP-eCLIP service please visit Eclipsebio . Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:100 - 1:200 Flow Cytometry (Fixed/Permeabilized): 1:400 eCLIP: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 78901 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity eIF4G2/p97 (D88B6) Rabbit Monoclonal Antibody detects endogenous levels of total eIF4G2/p97 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu450 of human eIF4G2/p97 protein. Background The initiation of translation is an important biological event and a variety of factors contribute to this process. Members of the eIF4 translation initiation factor family bind to the 5' m GTP mRNA cap and unwind the mRNA secondary structure (1,2). The amino-terminal portion of eIF4G physically associates with eIF4E to stimulate the binding of eIF4E to the mRNA cap structure (3). eIF4G also interacts with eIF3 and eIF4A and serves as an adaptor molecule in the eIF4 complex (4). Moreover, eIF4G plays a role in internal ribosomal entry site (IRES)-mediated initiation of translation (5,6). The eIF4G family includes eIF4G1 (eIF4GI), eIF4G2 (p97, DAP5 or NAT1), and eIF4G3 (eIF4GII) (7). These factors share a homologous sequence that provides for interaction with initiation factors eIF3 and eIF4A. Both eIF4G1 and eIF4G3 are involved in cap-dependent translation, while eIF4G2 plays a role in IRES-mediated translation of some genes during cell stress (7,8). Alternate Names AAG1; aging-associated protein 1; DAP-5; DAP5; Death-associated protein 5; eIF-4-gamma 2; eIF-4G 2; eIF4G 2; EIF4G2; Eukaryotic translation initiation factor 4 gamma 2; eukaryotic translation initiation factor 4 gamma, 2; eukaryotic translation initiation factor 4G-like 1; FLJ41344; IF4G2; NAT1; p97 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 97 Source/Isotype: Rabbit IgG