CST, 51765SF, CNPase (D83E10) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying CNPase. Validated for Western Blotting,Immunofluorescence (Frozen). Highly specific and rigorously validated in-house, CNPase (D83E10) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #51765) is ready to ship. Product Usage Information This product is the carrier free version of product #5664. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 5664 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity CNPase (D83E10) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total CNPase protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val81 of human CNPase protein. Background CNPase (2', 3'-cyclic nucleotide 3'-phosphodiesterase) catalyzes the hydrolysis of 2', 3'-cyclic nucleotides to produce 2'-nucleotides. The molecular function and native substrate of this nucleotide phosphodiesterase remains under investigation (1). High CNPase expression is seen in oligodendrocytes and Schwann cells as CNPase accounts for roughly 4% of the total myelin protein in the central nervous system (2). CNPase binds to tubulin heterodimers and plays a role in tubulin polymerization, and oligodendrocyte process outgrowth (3). Typical myelination is seen in CNPase knock-out mice, but they suffer severe neurodegeneration from axonal loss and oligodendrocytes display abnormal paranodal loop structure prior to axonal degeneration. Paranodal loops typically contact the axolemma in axon-glial signaling; neurodegeneration in CNPase knock-out mice is a secondary consequence of impaired cell-cell communication (4). Alternate Names 2', 3' cyclic nucleotide 3' phosphohydrolase; 2',3'-cyclic nucleotide 3' phosphodiesterase; 2',3'-cyclic-nucleotide 3'-phosphodiesterase; CN37; CNP; CNP1; CNPase Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 47 Source/Isotype: Rabbit IgG