CST, 51774SF, LAMP1 (D4O1S) Mouse Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying LAMP1. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, LAMP1 (D4O1S) Mouse Monoclonal Antibody (BSA and Azide Free) (CST #51774) is ready to ship. Product Usage Information This product is the carrier free version of product #15665. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 15665 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity LAMP1 (D4O1S) Mouse Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total LAMP1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a recombinant protein fragment of human LAMP1 protein. Background Lysosome-associated membrane protein 1 and 2 (LAMP1 and LAMP2) are two abundant lysosomal membrane proteins (1,2). Both are transmembrane proteins and are heavily glycosylated at the amino-terminal luminal side of the lysosomal inner leaflet, which protects the proteins from proteolysis (3). The carboxy terminus of LAMP1 is exposed to the cytoplasm and contains a tyrosine sorting motif that targets LAMP to lysosomal membranes (4). LAMP1 and LAMP2 are 37% homologous in their protein sequences. Both LAMP1 and LAMP2 are involved in regulating lysosomal motility during lysosome-phagosome fusion and cholesterol trafficking (5,6). Alternate Names CD107 antigen-like family member A; CD107a; LAMP-1; LAMP1; LAMPA; LGP120; lysosomal associated membrane protein 1; lysosomal-associated membrane protein 1; Lysosome-associated membrane glycoprotein 1; Lysosome-associated membrane protein 1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 42 (non-glycosylated), 90-120 (glycosylated) Source/Isotype: Mouse IgG1