CST, 51802S, NeuN (D4G4O) Rabbit Monoclonal Antibody (Biotinylated)

Monoclonal Antibody for studying NeuN. Validated for Western Blotting. Highly specific and rigorously validated in-house, NeuN (D4G4O) Rabbit Monoclonal Antibody (Biotinylated) (CST #51802) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 140 mM NaCl, 3 mM KCI, 10 mM sodium phosphate (pH 7.4) dibasic, 2 mM potassium phosphate monobasic, 2 mg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity NeuN (D4G4O) Rabbit Monoclonal Antibody (Biotinylated) recognizes endogenous levels of total NeuN protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human NeuN protein. Background Neuronal nuclei (NeuN, Fox-3, RBFOX3) is a nuclear protein expressed in most post-mitotic neurons of the central and peripheral nervous systems. NeuN is not detected in Purkinje cells, sympathetic ganglion cells, Cajal-Retzius cells, INL retinal cells, inferior olivary, or dentate nucleus neurons (1). This neuronal protein was originally identified by immunoreactivity with a monoclonal antibody also called NeuN. Using MS analysis, NeuN was later identified as the gene product. Fox-3 contains an RNA recognition motif and functions as a splicing regulator (2). Fox-3 regulates alternative splicing of NumB, promoting neuronal differentiation during development (3). Alternate Names FLJ56884; FLJ58356; fox-1 homolog C; Fox-3; FOX3; hCG_1776007; hexaribonucleotide binding protein 3; HRNBP3; NEUN; NeuN antigen; neuronal nuclei; Neuronal nuclei antigen; RBFOX3; RFOX3; RNA binding fox-1 homolog 3; RNA binding protein fox-1 homolog 3; RNA binding protein, fox-1 homolog 3 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 46-55 Source/Isotype: Rabbit IgG