CST, 51817P, ITK (E4X7M) Rabbit Monoclonal Antibody (Biotinylated)

Monoclonal Antibody for studying Itk. Validated for Western Blotting,Peptide ELISA (DELFIA). Highly specific and rigorously validated in-house, ITK (E4X7M) Rabbit Monoclonal Antibody (Biotinylated) (CST #51817) is ready to ship. Product Usage Information Biotinylated antibodies are ideal for immunoassay technologies and high-throughput ELISA platforms that require antibody pairs where both antibodies are from the same host. Platforms utilizing biotinylated antibodies include, but are not limited to, MSD, xMAP, Quanterix Simoa, AlphaLISA, AlphaScreen, HTRF, LANCE, and TR-FRET. Optimal dilutions/working concentrations should be determined by the end user. Please contact us if you require the antibody clone biotinylated at a different concentration, a carrier-free formulation, or a more customized packaging solution. Storage Supplied in 140 mM NaCl, 3 mM KCl, 10 mM sodium phosphate (pH 7.4) dibasic, 2 mM potassium phosphate monobasic, 2 mg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity ITK (E4X7M) Rabbit Monoclonal Antibody (Biotinylated) recognizes endogenous levels of total ITK protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ITK protein. Background Interleukin-2 inducible T cell kinase (Itk, Emt, or Tsk) is a member of the non-receptor protein tyrosine kinases. Family members of Itk include Tec, Btk, Rlk, and Bmx and are all defined by a common structure: an amino-terminal PH domain, a Tec-homology domain, and an SH3 and SH2 domain followed by a carboxy-terminal kinase domain (1). Tec, Rlk, and Itk are expressed in T cells and activated in response to T cell receptor (TCR) engagement. Data demonstrate that Itk functions in signal transduction downstream of TCR and activates PLCgamma1 and Erk. Lck directly activates Itk through phosphorylation in the conserved activation loop at Tyr511, and furthermore, Itk is autophosphorylated in the SH3 domain at Tyr180. Itk-Y180F is still capable of phosphorylating PLCgamma1 in contrast to Itk-Y511F, which has lost that function (2-3). Itk -/- mice show reduced lung inflammation, eosinophil infiltration, and mucous production in response to allergic asthma induction. Thus, Itk could become a desirable target for anti-asthmatic treatments (4). Alternate Names EMT; homolog of mouse T-cell itk/tsk; IL-2-inducible T-cell kinase; IL2 inducible T cell kinase; IL2-inducible T-cell kinase; Interleukin-2-inducible T-cell kinase; ITK; Kinase EMT; LPFS1; LYK; MGC126257; MGC126258; PSCTK2; T-cell-specific kinase; Tyrosine-protein kinase ITK/TSK; Tyrosine-protein kinase Lyk Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 72 Source/Isotype: Rabbit IgG