CST, 51865SF, Phospho-STING (Ser365) (D1C4T) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying STING (Ser365) phosphate mouse. Validated for Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, Phospho-STING (Ser365) (D1C4T) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #51865) is ready to ship. Product Usage Information This product is the carrier free version of product #62912. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 62912 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Phospho-STING (Ser365) (D1C4T) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of STING protein only when phosphorylated at Ser365. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser365 of mouse STING protein. Background Stimulator of interferon genes (STING, TMEM173, MITA) is a transmembrane adaptor protein that is a critical component of the cellular innate immune response to pathogenic cytoplasmic DNA (1,2). STING is a ubiquitously expressed protein found predominantly in the ER (1). The enzyme cGAMP synthase (cGAS) produces the second messenger cyclic-GMP-AMP (cGAMP) in response to cytoplasmic DNA (3,4). cGAMP binds and activates STING (3,4). In addition, detection of cytoplasmic DNA by nucleic acid sensors, including DDX41 or IFI16, results in STING activation (5,6). Following activation, STING translocates with TBK1 to perinuclear endosomes (7). The TBK1 kinase phosphorylates and activates interferon regulatory factors (IRFs) and NF-κB, which leads to the induction of type I interferon and other immune response genes (1,2,7). Following binding of cyclic dinucleotides, STING is phosphorylated by TBK1 at Ser366 (Ser365 in mouse), leading to IRF-3 activation and type I interferon upregulation (8). Alternate Names 2610307O08Rik; Endoplasmic reticulum interferon stimulator; ERIS; Mediator of IRF3 activation; Mita; mitochondrial mediator of IRF3 activation; MMITA; Mpys; mSTING; Stimulator of interferon genes protein; stimulator of interferon protein; stimulator of interferon response cGAMP interactor 1; Sting; STING-beta; Sting1; TM173; Tmem173; Transmembrane protein 173 Specification REACTIVITY: M SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG