CST, 51872T, TBK1/NAK (E9H5S) Mouse Monoclonal Antibody

Monoclonal Antibody for studying TBK1. Validated for Western Blotting,Fluorescent Western,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, TBK1/NAK (E9H5S) Mouse Monoclonal Antibody (CST #51872) is ready to ship. Product Usage Information Western Blotting: 1:1000 Fluorescent Western: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 56401 . Protocol Available protocols: Western Blotting, Fluorescent Western, Immunoprecipitation Specificity / Sensitivity TBK1/NAK (E9H5S) Mouse Monoclonal Antibody recognizes endogenous levels of total TBK1/NAK protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu540 of human TBK1/NAK protein. Background TBK1 (TANK-binding kinase 1)/NAK (NF-κB activating kinase) is an IκB kinase (IKK)-activating kinase and can activate IKK through direct phosphorylation (1). TBK1 was identified through association with the TRAF binding protein, TANK, and found to function upstream of NIK and IKK in the activation of NF-κB (2). TBK1 induces IκB degradation and NF-κB activity through IKKβ. TBK1 may mediate IKK and NF-κB activation in response to growth factors that stimulate PKCε activity (1). TBK1 plays a pivotal role in the activation of IRF3 in the innate immune response (3). Alternate Names FLJ11330; FTDALS4; IIAE8; NAK; NF-kappa-B-activating kinase; NF-kB-activating kinase; Serine/threonine-protein kinase TBK1; T2K; TANK binding kinase 1; TANK-binding kinase 1; TBK1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 84 Source/Isotype: Mouse IgG1