CST, 51875S, Myc-Tag (71D10) Rabbit Monoclonal Antibody (Pacific Blue™ Conjugate)

Monoclonal Antibody for studying . Validated for Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, Myc-Tag (71D10) Rabbit Monoclonal Antibody (Pacific Blue^™ Conjugate) (CST #51875) is ready to ship. Product Usage Information Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Myc-Tag (71D10) Rabbit Monoclonal Antibody (Pacific Blue™ Conjugate) detects exogenously expressed proteins containing the Myc epitope tag. This antibody conjugate recognizes the Myc tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells. This antibody conjugate detects exogenously expressed Myc-tagged proteins in cells expressed under a CMV promoter. Expression under other promoters has not been evaluated. This antibody conjugate may cross-react with c-Myc protein by flow cytometry and immunofluorescence assays. Species Reactivity: All Species Expected Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues 410-419 of human c-Myc (EQKLISEEDL). Background Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties. The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect, and mammalian cell systems (1). Specification REACTIVITY: All SENSITIVITY: Transfected Only Source/Isotype: Rabbit IgG