CST, 51888T, GRAP (F9U7T) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying GRAP. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, GRAP (F9U7T) Rabbit Monoclonal Antibody (CST #51888) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity GRAP (F9U7T) Rabbit Monoclonal Antibody recognizes endogenous levels of total GRAP protein. This antibody does not cross-react with GRB2 protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human GRAP protein. Background GRB2-related adaptor protein (GRAP) is a member of the GRB2 family of signaling adaptor proteins, and its expression is largely restricted to lymphoid tissues (1,2). Similar to the structure of other members of the GRB2 family of adaptor proteins, GRAP contains a central SH2 domain flanked on either side by SH3 domains that allow for it to directly associate with other signaling molecules (1). Research studies have suggested that, unlike GRB2, GRAP may hinder T cell effector functions by disrupting Ras/MAPK signaling downstream of the T cell receptor signaling complex (3). Alternate Names DFNB114; GRAP; GRB2 related adaptor protein; GRB2-related adapter protein; GRB2-related adaptor protein; growth factor receptor-bound protein 2-related adaptor protein; MGC64880; Q13588 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 25 Source/Isotype: Rabbit IgG