CST, 5199P, Phospho-eIF2 alpha (Ser51) (D9G8) Rabbit Monoclonal Antibody (Biotinylated)

Monoclonal Antibody for studying EIF2S1/eIF2-alpha (Ser52) phosphate. Validated for Western Blotting,Peptide ELISA (DELFIA). Highly specific and rigorously validated in-house, Phospho-eIF2 alpha (Ser51) (D9G8) Rabbit Monoclonal Antibody (Biotinylated) (CST #5199) is ready to ship. Product Usage Information Biotinylated antibodies are ideal for immunoassay technologies and high-throughput ELISA platforms that require antibody pairs where both antibodies are from the same host. Platforms utilizing biotinylated antibodies include, but are not limited to, MSD, xMAP, Quanterix Simoa, AlphaLISA, AlphaScreen, HTRF, LANCE, and TR-FRET. Optimal dilutions/working concentrations should be determined by the end user. Please contact us if you require the antibody clone biotinylated at a different concentration, a carrier-free formulation, or a more customized packaging solution. Storage Supplied in 140 mM NaCl, 3 mM KCl, 10 mM sodium phosphate (pH 7.4) dibasic, 2 mM potassium phosphate monobasic, 2 mg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-eIF2 alpha (Ser51) (D9G8) Rabbit Monoclonal Antibody (Biotinylated) detects endogenous eIF2α protein only when phosphorylated at Ser51. This antibody does not recognize elF2α phosphorylated at other sites. Human eIF2alpha residue Ser52 historically has been referenced as Ser51. Species Reactivity: Human, Mouse, Rat, Monkey, D. melanogaster Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser51 of human eIF2α protein. Background Phosphorylation of the eukaryotic initiation factor 2 (eIF2) α subunit is a well-documented mechanism to downregulate protein synthesis under a variety of stress conditions. eIF2 binds GTP and Met-tRNAi and transfers Met-tRNA to the 40S subunit to form the 43S preinitiation complex (1,2). eIF2 promotes a new round of translation initiation by exchanging GDP for GTP, a reaction catalyzed by eIF2B (1,2). Kinases that are activated by viral infection (PKR), endoplasmic reticulum stress (PERK/PEK), amino acid deprivation (GCN2), or heme deficiency (HRI) can phosphorylate the α subunit of eIF2 (3,4). This phosphorylation stabilizes the eIF2-GDP-eIF2B complex and inhibits the turnover of eIF2B. Induction of PKR by IFN-γ and TNF-α induces potent phosphorylation of eIF2α at Ser51 (5,6). Alternate Names EIF-2; eIF-2-alpha; eIF-2A; eIF-2alpha; EIF2; eIF2-alpha; EIF2A; EIF2S1; Eukaryotic translation initiation factor 2 subunit 1; Eukaryotic translation initiation factor 2 subunit alpha; eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa; IF2A Specification REACTIVITY: H M R Mk Dm SENSITIVITY: Endogenous MW (kDa): 38 Source/Isotype: Rabbit IgG