CST, 52113S, SOCS3 (D6E1T) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SOCS3. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, SOCS3 (D6E1T) Rabbit Monoclonal Antibody (CST #52113) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity SOCS3 (D6E1T) Rabbit Monoclonal Antibody recognizes endogenous levels of total SOCS3 protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to human SOCS3 protein. Background The suppressor of cytokine signaling (SOCS) family members are negative regulators of cytokine signal transduction that inhibit the Jak/Stat pathway (1-3). The SOCS family consists of at least 8 members including the originally identified cytokine-inducible SH2-containing protein (CIS1), as well as SOCS1-7. Each SOCS family member contains a central SH2 domain and a conserved carboxy-terminal motif designated as the SOCS box. These proteins are important regulators of cytokine signaling, proliferation, differentiation, and immune responses. Low levels of SOCS3 are observed in lung, spleen, and thymus and, like other SOCS family members, its expression is rapidly induced by a number of factors including interleukins, EPO, IFN-γ, CSF, and TNF-α (4). SOCS3 uses its SH2 domain to bind activated Jaks and their cognate receptors to provide negative feedback inhibition. In addition to the initially described inducers of SOCS3 expression, subsequent studies have described SOCS3-mediated negative feedback inhibition for leptin (5), GH (6), chemokine receptors (7), insulin (8), and certain pathogens (9,10). SOCS3 deletion results in embryonic lethality with placental insufficiency (11). SOCS3 signaling has been linked pathologically to allergic responses (12), inflammatory disease (13), endotoxic shock (14), wound repair (15), and obesity (16,17). Alternate Names ATOD4; CIS-3; CIS3; Cish3; cytokine-induced SH2 protein 3; Cytokine-inducible SH2 protein 3; MGC71791; SOCS-3; SOCS3; SSI-3; SSI3; STAT-induced STAT inhibitor 3; Suppressor of cytokine signaling 3 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 28 Source/Isotype: Rabbit IgG