CST, 52141T, MAP2A/MAP2B (F5H5D) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MAP2 mouse. Validated for WB,WB,IHC,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, MAP2A/MAP2B (F5H5D) Rabbit Monoclonal Antibody (CST #52141) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunohistochemistry (Paraffin): 1:200 - 1:800 Immunofluorescence (Frozen): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen) Specificity / Sensitivity MAP2A/MAP2B (F5H5D) Rabbit Monoclonal Antibody recognizes endogenous levels of total MAP2A/MAP2B protein. This antibody does not cross-react with MAP2C or MAP2D isoforms. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val332 of mouse MAP2A/MAP2B protein. Background Microtubule-associated protein 2 (MAP2) is a neuronal phosphoprotein that regulates the structure and stability of microtubules, neuronal morphogenesis, cytoskeleton dynamics, and organelle trafficking in axons and dendrites (1). Multiple MAP2 isoforms are expressed in neurons, including high molecular weight MAP2A and MAP2B (280 and 270 kDa), and low molecular weight MAP2C and MAP2D (70 and 75 kDa). Phosphorylation of MAP2 modulates its association with the cytoskeleton and is developmentally regulated. GSK-3 and p44/42 MAP kinase phosphorylate MAP2 at Ser136, Thr1620, and Thr1623 (2,3). Phosphorylation at Thr1620/1623 by GSK-3 inhibits MAP2 association with microtubules and microtubule stability (3). Alternate Names G1-397-34; MAP; MAP-2; Map2; Microtubule-associated protein 2; Mta; Mtap-2; Mtap2; OTTMUSP00000017688; OTTMUSP00000017689; OTTMUSP00000017691; repro4 Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 280 Source/Isotype: Rabbit IgG