CST, 5214S, T-bet/TBX21 (V365) Antibody

Polyclonal Antibody for studying TBX21. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, T-bet/TBX21 (V365) Antibody (CST #5214) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity T-bet/TBX21 (V365) Antibody detects endogenous levels of total T-bet protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val365 of human T-bet/TBX21 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background The gene family consists of transcription factors characterized by a related DNA-binding domain (T-box) of approximately 200 amino acids (1,2). The genes exhibit diverse temporal and spatial patterns in the developing embryo. Studies have demonstrated members of this family play crucial roles during embryogenesis in a wide range of organisms by regulating cell fate decisions to establish the early body plan and to regulate later processes underlying organogenesis (3-5). Mutations in genes are associated with many developmental defects (6). Recent studies also indicate potential roles in cancer by members of the T-box family (7-9). T-bet, also as known as TBX21, plays a critical role in development and maintenance of type 1 helper T (Th1) and T-bet deficient mice display impaired Th1 differentiation (10,11). Alternate Names T-bet; T-box 21; T-box expressed in T cells; T-box protein 21; T-box transcription factor 21; T-box transcription factor TBX21; T-cell-specific T-box transcription factor T-bet; T-PET; TBET; TBLYM; TBX21; Transcription factor TBLYM Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 65 SOURCE: Rabbit