CST, 52238T, Ornithine Decarboxylase/ODC (E5Q5U) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ODC1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Ornithine Decarboxylase/ODC (E5Q5U) Rabbit Monoclonal Antibody (CST #52238) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Ornithine Decarboxylase/ODC (E5Q5U) Rabbit Monoclonal Antibody recognizes endogenous levels of total ornithine decarboxylase/ODC protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a recombinant protein of human ornithine decarboxylase/ODC protein. Background Ornithine decarboxylase (ODC) is a rate-limiting enzyme in polyamine biosynthesis. Studies found that ODC and polyamine levels are often increased, contributing to cancer cell proliferation (1). Deficiency of the ODC enzyme suppresses acute myeloid leukemia cell proliferation (2). In addition, oncogene products YAP and TAZ activate transcription of the gene, which encodes the ODC enzyme, leading to more polyamine biosynthesis. Enhanced polyamine levels mediate tumor growth driven by YAP and TAZ via a polyamine-eIF5A hypusination-LSD1 axis (3). Furthermore, continual efferocytosis of apoptotic cells by macrophages is promoted by converting arginine and ornithine, both from apoptotic cells, to putrescine by arginase-1 and ODC in macrophages. Putrescine enhances subsequent rounds of efferocytosis (4). Alternate Names DCOR; ODC; ODC1; Ornithine decarboxylase; ornithine decarboxylase 1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 50 Source/Isotype: Rabbit IgG