CST, 52267S, F4/80 (BM8.1) Rat Monoclonal Antibody (FITC Conjugate)

Monoclonal Antibody for studying EMR1 mouse. Validated for Flow Cytometry (Fixed/Permeabilized),Flow Cytometry (Live). Highly specific and rigorously validated in-house, F4/80 (BM8.1) Rat Monoclonal Antibody (FITC Conjugate) (CST #52267) is ready to ship. Product Usage Information For optimal flow cytometry results, we recommend 0.5μg of antibody per test. Flow Cytometry (Fixed/Permeabilized): 1:100 Flow Cytometry (Live): 1:100 Storage Supplied in 10 mM NaH 2 PO 4 , 150 mM NaCl, 0.09% NaN 3 , 0.1% gelatin, pH 7.2. This product is stable for 12 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Flow Cytometry (Fixed/Permeabilized), Flow Cytometry (Live) Specificity / Sensitivity F4/80 (BM8.1) Rat Monoclonal Antibody (FITC Conjugate) recognizes endogenous levels of total F4/80 protein. This antibody conjugate detects an epitope within the extracellular domain. Species Reactivity: Mouse Source / Purification This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. Background F4/80 (EMR1) is a heavily glycosylated G-protein-coupled receptor and is a well-established marker for mouse macrophages (1-3). Expression of F4/80 has also been observed in microglia and subset populations of dendritic cells (4). BM8.1 is widely used together with antibodies to CD115 (c-Fms), CD11b, and CD11c to identify myeloid/macrophage cells by flow cytometry. Alternate Names Adgre1; Adhesion G protein-coupled receptor E1; AGRE1; Cell surface glycoprotein F4/80; DD7A5-; DD7A5-7; EGF-like module containing, mucin-like, hormone receptor-like sequence 1; EGF-like module receptor 1; EGF-like module-containing mucin-like hormone receptor-like 1; EGF-TM7; Emr; Emr1; EMR1 hormone receptor; F4/80; Gpf480; Ly7; Ly71; lymphocyte antigen 71; TM7L; TM7LN3 Specification REACTIVITY: M SENSITIVITY: Endogenous Source/Isotype: Rat IgG2b kappa