CST, 52293T, USP22 (F4M4V) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying USP22. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, USP22 (F4M4V) Rabbit Monoclonal Antibody (CST #52293) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:100 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity USP22 (F4M4V) Rabbit Monoclonal Antibody recognizes endogenous levels of total USP22 protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu457 of human USP22 protein. Background Ubiquitin-specific-processing protease 22 (USP22) is a deubiquitinating enzyme that, along with TXN7, ATXN7L3, and ENY2, forms the deubiquitination module of the human SAGA complex (1,2). SAGA is capable of deubiquitinating H2A and H2B in opposition to polycomb activity (3,4). In addition to histone targets, USP22 deubiquitinates and stabilizes SirT1, which in turn deacetylates and suppresses p53 to repress apoptosis (5). Regulation of SirT1 by USP22 has also been shown to affect STAT signaling (6). USP22 plays a role in double-stranded break repair and antibody class switching (7,8). USP22 has also been shown to occupy the SOX2 promoter, blocking transcription and thus regulating pluripotency (9). Many cancers have elevated levels of USP22, which can promote cancer progression and metastasis (10-12). Alternate Names Deubiquitinating enzyme 22; KIAA1063; Ubiquitin carboxyl-terminal hydrolase 22; ubiquitin specific peptidase 22; ubiquitin specific peptidase 3-like; ubiquitin specific protease 22; Ubiquitin thioesterase 22; ubiquitin thiolesterase 22; ubiquitin-specific processing protease 22; Ubiquitin-specific-processing protease 22; UBP22; USP22; USP3L Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 60 Source/Isotype: Rabbit IgG