CST, 52305SF, LRRC15 (E4X8J) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying LRRC15. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, LRRC15 (E4X8J) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #52305) is ready to ship. Product Usage Information This product is the carrier free version of product #50546. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 50546 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity LRRC15 (E4X8J) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total LRRC15 protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu100 of human LRRC15 protein. Background LRRC15 (Leucine-rich repeat-containing protein 15, LIB) is a transmembrane protein belonging to the LRR family of proteins involved in cell-cell and cell-ECM (extracellular matrix) interactions (1-4). It has been identified as a marker for TGF-β-driven CAFs (cancer-associated fibroblasts), which are associated with immunotherapy resistance (3,4). LRRC15 is highly expressed in multiple solid tumor types, with low expression in normal tissues, making it a good therapeutic candidate for cancers with high stromal content, such as pancreatic cancer, non-small cell lung cancer (NSCLC), and triple-negative breast cancer (3,4). Alternate Names hLib; leucine rich repeat containing 15; leucine-rich repeat protein induced by beta amyloid; Leucine-rich repeat protein induced by beta-amyloid homolog; Leucine-rich repeat-containing protein 15; LIB; LRC15; LRRC15 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 80-100 Source/Isotype: Rabbit IgG