CST, 52339S, GSTM1 Antibody

Polyclonal Antibody for studying GSTM1. Validated for Western Blotting. Highly specific and rigorously validated in-house, GSTM1 Antibody (CST #52339) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity GSTM1 Antibody recognizes endogenous levels of total GSTM1 protein. Species Reactivity: Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu135 of human GSTM1 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Glutathione S-transferase Mu 1, encoded by GSTM1, belongs to the superfamily of glutathione-S transferases (GSTs). It is a phase 2 enzyme involved in the inactivation of reactive oxygen species (ROS) and metabolism of xenobiotics, using glutathione and generating oxidized glutathione (1). Polymorphisms of the gene have been associated with nasopharyngeal cancer, laryngeal cancer, colorectal cancer, hypertension, and chronic kidney disease (1-3). In the brain, GSTM1 contributes to astrocyte-mediated enhancement of microglia activation during brain inflammation as it is required for nuclear factor κB (NF-κB) activation and proinflammatory mediator production (4). Alternate Names glutathione S-alkyltransferase; glutathione S-aralkyltransferase; glutathione S-aryltransferase; glutathione S-transferase M1; Glutathione S-transferase Mu 1; GST class-mu 1; GST HB subunit 4; GST1; GSTM1; GSTM1-1; GSTM1a-1a; GSTM1b-1b; GTH4; GTM1; H-B; HB subunit 4; MGC26563; MU; MU-1; S-(hydroxyalkyl)glutathione lyase Specification REACTIVITY: M R SENSITIVITY: Endogenous MW (kDa): 25 SOURCE: Rabbit