CST, 52345W2, CD8 alpha (D8A8Y) Rabbit Monoclonal Antibody (SignalFlex™ mFluor™ Red 780 Conjugate)

Monoclonal Antibody for studying CD8A. Highly specific and rigorously validated in-house, CD8 alpha (D8A8Y) Rabbit Monoclonal Antibody (SignalFlex^™ mFluor^™ Red 780 Conjugate) (CST #52345) is ready to ship. Product Usage Information SignalFlex™ conjugates are produced using highly validated Cell Signaling Technology ® primary antibodies and conjugation methods that have been rigorously tested, ensuring high-quality conjugates and lot-to-lot consistency. These conjugates are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. However, they are not tested on specific assays. Optimal dilutions/concentrations should be determined by the end user. When performing flow cytometry, we recommend using an isotype control conjugate at the same concentration as the antibody conjugate. Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Specificity / Sensitivity CD8α (D8A8Y) Rabbit mAb (SignalFlex™ mFluor™ Red 780 Conjugate) recognizes endogenous levels of total CD8α protein. Monkey species reactivity is Immunohistochemistry (Paraffin) only. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human CD8α protein. Background Cluster of Differentiation 8 (CD8) is a disulphide-linked heterodimer consisting of the unrelated α and β subunits. Each subunit is a glycoprotein composed of a single extracellular Ig-like domain, a polypeptide linker, a transmembrane part and a short cytoplasmic tail. On T cells, CD8 is the coreceptor for the T cell receptor (TCR), and these two distinct structures recognize the Antigen-Major Histocompatibility Complex (MHC). Specifically, the Ig-like domain of CD8α interacts with the α3-domain of the MHC class I molecule. CD8 ensures specificity of the TCR-antigen interaction, prolongs the contact between the T cell and the antigen presenting cell, and the α chain recruits the tyrosine kinase Lck, which is essential for T cell activation (1). Alternate Names CD8; CD8 antigen alpha polypeptide; CD8 antigen, alpha polypeptide (p32); CD8A; CD8a molecule; Leu2; Leu2 T-lymphocyte antigen; MAL; OKT8 T-cell antigen; p32; T cell co-receptor; T-cell antigen Leu2; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2; T8 T-cell antigen Specification REACTIVITY: H Mk SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG