CST, 52405S, TMOD3 (F9M4D) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TMOD3. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, TMOD3 (F9M4D) Rabbit Monoclonal Antibody (CST #52405) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:1600 - 1:6400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity TMOD3 (F9M4D) Rabbit Monoclonal Antibody recognizes endogenous levels of total TMOD3 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys101 of human TMOD3 protein. Background Tropomodulin-1 (TMOD1) belongs to a conserved family of cytoskeletal proteins (TMOD1-4) that play an important role in modulating actin cytoskeleton dynamics. TMOD proteins function as actin capping proteins, which stabilize actin filaments by inhibiting both elongation and depolymerization (1). While many proteins have been identified that cap the rapidly growing barbed end of actin filaments, TMODs are the only proteins thus far identified that cap the slowly growing pointed end (2). A research study in triple-negative breast cancer cells identified TMOD1 as a target of NF-κB signaling, and showed that increased TMOD1 expression was associated with enhanced tumor growth in a mouse xenograft model (3). Molecular expression of was also identified as part of a unique gene expression signature that could discriminate ALK-negative anaplastic large-cell lymphoma from other malignancy subtypes (4). TMOD3 is widely expressed and, like other family members, binds tropomyosin and actin filaments (F-actin). This functions in the capping process (5). However, unlike other family members, TMOD3 is also able to bind to actin monomer (6). TMOD3 localizes to the lateral cell membrane in polarized epithelial cells and regulates cell shape (7). Loss of TMOD3 in mice leads to embryonic lethality with an impaired erythroid differential in the fetal liver (8). TMOD3 is uniquely phosphorylated by Akt2 upon insulin stimulation and regulates Glut4 trafficking through actin remodeling (9). Expression of TMOD3 may promote tumor progression, including liver cancer (10). Studies have also found a ferroptosis-related gene signature in lung adenocarcinoma that includes TMOD3 (11). Alternate Names TMOD3; tropomodulin 3; tropomodulin 3 (ubiquitous); Tropomodulin-3; U-Tmod; Ubiquitous tropomodulin; UTMOD Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 40 Source/Isotype: Rabbit IgG