CST, 5240S, Phospho-ENSA (Ser67)/ARPP19 (Ser62) Antibody

Polyclonal Antibody for studying ARPP-19 (Ser62) phosphate/ENSA (Ser67) phosphate. Validated for Western Blotting. Highly specific and rigorously validated in-house, Phospho-ENSA (Ser67)/ARPP19 (Ser62) Antibody (CST #5240) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-ENSA (Ser67)/ARPP19 (Ser62) Antibody recognizes endogenous levels of ENSA and ARPP19 proteins only when phosphorylated at Ser67 and Ser62, respectively. Species Reactivity: Human, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser67/Ser62 of human ENSA/ARPP19 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Mitotic control is important for normal growth, development, and maintenance of all eukaryotic cells. Research studies have demonstrated that inappropriate control of mitosis can lead to genomic instability and cancer (reviewed in 1,2). A regulator of mitosis, Greatwall kinase (Gwl), was first identified in (3). Subsequent studies showed that, based on sequence homology and function, microtubule-associated serine/threonine kinase-like (MASTL) is the human ortholog of Gwl (4). Regulation of MASTL/Gwl activation has been shown to be critical for the correct timing of mitosis. Research studies have shown that Gwl is activated by hyperphosphorylation (5). The phosphorylation of human Gwl at Thr194 and Thr207 by active cyclin B1-cdc2 leads to possible autophosphorylation at Ser875 (Ser883 in ), which stabilizes the kinase. Activated Gwl phosphorylates α-Endosulfine (ENSA) and cAMP-regulated phosphoprotein 19 (ARPP19) at Ser67 and Ser62, respectively. Phosphorylated ENSA and ARPP19 inhibit the activity of the B55 subunit-associated form of protein phosphatase 2A (PP2A-B55), allowing for complete phosphorylation of mitotic substrates by cyclin B1-cdc2 and mitotic entry. When Gwl is inactivated, PP2A-B55 reactivates, which leads to dephosphorylation of cyclin B1-cdc2 and mitotic exit (5,6, reviewed in 7). Alternate Names Alpha-endosulfine; ARP19; ARPP-16; ARPP-19; ARPP-19e; ARPP16; ARPP19; cAMP regulated phosphoprotein 19; cAMP-regulated phosphoprotein 19; cAMP-regulated phosphoprotein 19kDa; cAMP-regulated phosphoprotein, 19kDa; cyclic AMP phosphoprotein, 19 kD; endosulfine alpha; endosulfine alpha-like; ENSA; ENSAL; FLJ41622; MGC4319; MGC78563; MGC8394 Specification REACTIVITY: H R SENSITIVITY: Endogenous MW (kDa): 15 SOURCE: Rabbit