CST, 52445S, HGF beta (D6S7D) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying HGF beta subunit. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, HGF beta (D6S7D) Rabbit Monoclonal Antibody (CST #52445) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 57408 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity HGF beta (D6S7D) Rabbit Monoclonal Antibody recognizes endogenous levels of total HGF protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human HGF protein. Background The Hepatocyte Growth Factor (HGF, also known as Scatter Factor) was initially discovered as a mitogenic cytokine that induced hepatocyte replication and proliferation (1-3). HGF is produced by stromal cells where it is processed by extracellular serine proteases into a heterodimer consisting of alpha and beta subunits (4). Through activation of its receptor, cMET, HGF has a wide range of effects beyond hepatocytes that includes angiogenesis, epithelial cell proliferation and morphogenesis, and tissue protection and regeneration (5). The HGF-cMET axis has been associated with several diseases, including cancer, where HGF has been shown to promote invasion, metastasis, and drug resistance (6,7). These research studies suggest that HGF is a potential diagnostic and therapeutic target. Alternate Names DFNB39; F-TCF; fibroblast-derived tumor cytotoxic factor; Hepatocyte growth factor; hepatocyte growth factor (hepapoietin A; Hepatocyte growth factor alpha chain; Hepatocyte growth factor beta chain; Hepatopoeitin-A; Hepatopoietin-A; HGF; HGFB; HPTA; lung fibroblast-derived mitogen; Scatter factor; scatter factor); SF Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 35, 85 Source/Isotype: Rabbit IgG