CST, 5245S, STAMBP Antibody

Polyclonal Antibody for studying STAMBP. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, STAMBP Antibody (CST #5245) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity STAMBP Antibody detects endogenous levels of total STAMBP protein. Species Reactivity: Human, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly194 of human STAMBP protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Protein ubiquitination and deubiquitination is a reversible process catalyzed by ubiquitinating enzymes (UBEs) and deubiquitinating enzymes (1,2). Deubiquitinating enzymes (DUBs) function as ubiquitin-specific proteases and can be divided into five subfamilies based on catalytic domain structure. At least 14 members of the JAMM ubiquitin protease subfamily have been identified, including signal transducing adaptor molecule (STAM) binding protein (3). STAM-binding protein (STAMBP or AMSH) is an endosomal DUB that preferentially displays ubiquitin isopeptidase activity toward K63-linked chains, which is critically dependent upon its interaction with STAM (4,5). STAMBP interacts with the STAM adaptor protein and becomes integrated into the multivesicular body sorting machinery to help regulate endosomal trafficking and receptor tyrosine kinase stability by deubiquitining target proteins (4,6). Evidence indicates that endosomal STAMBP antagonizes the ubiquitin-dependent trafficking of EGFR to the lysosomal compartment (7). Alternate Names AMSH; Associated molecule with the SH3 domain of STAM; Endosome-associated ubiquitin isopeptidase; MGC126516; MGC126518; MICCAP; STABP; STAM binding protein; STAM-binding protein; STAMBP; testicular secretory protein Li 54 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 50 SOURCE: Rabbit