CST, 52474S, Bak (D4E4) Rabbit Monoclonal Antibody (Alexa Fluor® 488 Conjugate)

Monoclonal Antibody for studying Bak1. Validated for Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, Bak (D4E4) Rabbit Monoclonal Antibody (Alexa Fluor^® 488 Conjugate) (CST #52474) is ready to ship. Product Usage Information Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Bak (D4E4) Rabbit Monoclonal Antibody (Alexa Fluor Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly75 of human Bak protein. Background Bak is a proapoptotic member of the Bcl-2 family (1). This protein is located on the outer membrane of mitochondria and is an essential component for transduction of apoptotic signals through the mitochondrial pathway (2,3). Upon apoptotic stimulation, an upstream stimulator like truncated BID (tBID) induces conformational changes in Bak to form oligomer channels in the mitochondrial membrane for cytochrome c release. The release of cytochrome c to the cytosol activates the caspase-9 pathway and eventually leads to cell death (4,5). Alternate Names Apoptosis regulator BAK; BAK; BAK-LIKE; BAK1; Bcl-2 homologous antagonist/killer; Bcl-2-like protein 7; BCL2 antagonist/killer 1; BCL2-antagonist/killer 1; Bcl2-L-7; BCL2-like 7 protein; BCL2L7; CDN1; MGC117255; MGC3887; pro-apoptotic protein BAK Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG