CST, 5247S, COX IV (3E11) Rabbit Monoclonal Antibody (HRP Conjugate)

Monoclonal Antibody for studying COX IV. Validated for Western Blotting. Highly specific and rigorously validated in-house, COX IV (3E11) Rabbit Monoclonal Antibody (HRP Conjugate) (CST #5247) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 140 mM NaCl, 3 mM KCI, 10 mM sodium phosphate (pH 7.4) dibasic, 2 mM potassium phosphate monobasic, 2 mg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity COX IV (3E11) Rabbit Monoclonal Antibody (HRP Conjugate) detects endogenous levels of total COX IV protein. Species Reactivity: Human, Rat, Monkey, Zebrafish, Bovine, Pig Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys29 of human COX IV. Background Cytochrome c oxidase (COX) is a hetero-oligomeric enzyme consisting of 13 subunits localized to the inner mitochondrial membrane (1-3). It is the terminal enzyme complex in the respiratory chain, catalyzing the reduction of molecular oxygen to water coupled to the translocation of protons across the mitochondrial inner membrane to drive ATP synthesis. The three largest subunits forming the catalytic core are encoded by mitochondrial DNA, while the other smaller subunits, including COX IV, are nuclear-encoded. Research studies have shown that deficiency in COX activity correlates with a number of human diseases (4). The COX IV antibody can be used effectively as a mitochondrial loading control in cell-based research assays. Alternate Names COX IV-1; COX4; COX4-1; COX41; COX4I1; COXIV; COXIV-1; Cytochrome c oxidase polypeptide IV; Cytochrome c oxidase subunit 4 isoform 1, mitochondrial; cytochrome c oxidase subunit 4I1; cytochrome c oxidase subunit IV; Cytochrome c oxidase subunit IV isoform 1; MGC72016 Specification REACTIVITY: H R Mk Z B Pg SENSITIVITY: Endogenous MW (kDa): 17 Source/Isotype: Rabbit IgG