CST, 52485T, DCAF16 (F8E1P) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying DCAF16. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, DCAF16 (F8E1P) Rabbit Monoclonal Antibody (CST #52485) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity DCAF16 (F8E1P) Rabbit Monoclonal Antibody recognizes endogenous levels of total DCAF16 protein. This antibody may cross-react with proteins of unknown origin around 50 kDa and 100 kDa. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human DCAF16 protein. Background The CUL4-DDB1 ubiquitin ligase complex mediates the degradation of target proteins involved in various cellular processes, including DNA damage repair, cell cycle progression, transcription, and development (1-5). DDB1- and CUL4-associated factor 16 (DCAF16) is an E3 ubiquitin ligase that, like other members of the DCAF family, functions as a substrate receptor to recruit the CUL4-DDB1 complex to target proteins for ubiquitination (1,6). DCAF16 is often studied in the context of targeted protein degradation (TPD), where it is utilized to specifically mark disease-associated nuclear proteins for ubiquitination and removal (7). For instance, DCAF16 was found to mediate degradation of FKBP12 and BRD4 protein following treatment with heterobifunctional degrader compounds (6). Alternate Names C4orf30; DCA16; DCAF16; DDB1 and CUL4 associated factor 16; DDB1- and CUL4-associated factor 16; FLJ20280; MGC126765; MGC126767 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 25 Source/Isotype: Rabbit IgG