CST, 52501SF, eIF4G (C45A4) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying eIF4G. Validated for WB,IHC,IF,F. Highly specific and rigorously validated in-house, eIF4G (C45A4) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #52501) is ready to ship. Product Usage Information This product is the carrier free version of product #2469. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 2469 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity eIF4G (C45A4) Rabbit Monoclonal Antibody (BSA and Azide Free) detects endogenous levels of total eIF4G protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification eIF4G (C45A4) Rabbit mAb is produced by immunizing rabbits with a synthetic peptide corresponding to residues surrounding Gly188 of human eIF4G. Background The initiation of translation is an important biological event and a variety of factors contribute to this process. Members of the eIF4 translation initiation factor family bind to the 5' m GTP mRNA cap and unwind the mRNA secondary structure (1,2). The amino-terminal portion of eIF4G physically associates with eIF4E to stimulate the binding of eIF4E to the mRNA cap structure (3). eIF4G also interacts with eIF3 and eIF4A and serves as an adaptor molecule in the eIF4 complex (4). Moreover, eIF4G plays a role in internal ribosomal entry site (IRES)-mediated initiation of translation (5,6). The eIF4G family includes eIF4G1 (eIF4GI), eIF4G2 (p97, DAP5 or NAT1), and eIF4G3 (eIF4GII) (7). These factors share a homologous sequence that provides for interaction with initiation factors eIF3 and eIF4A. Both eIF4G1 and eIF4G3 are involved in cap-dependent translation, while eIF4G2 plays a role in IRES-mediated translation of some genes during cell stress (7,8). Alternate Names DKFZp686A1451; eIF-4-gamma 1; eIF-4G 1; eIF-4G1; EIF4-gamma; EIF4F; EIF4G; EIF4G1; EIF4GI; eucaryotic translation initiation factor 4G; Eukaryotic translation initiation factor 4 gamma 1; eukaryotic translation initiation factor 4 gamma, 1; IF4G1; p220; PARK18 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 220 Source/Isotype: Rabbit