CST, 5250S, Renin Antibody

Polyclonal Antibody for studying renin. Validated for Western Blotting. Highly specific and rigorously validated in-house, Renin Antibody (CST #5250) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Renin Antibody detects transfected levels of human Renin protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu308 of human renin. Antibodies are purified by protein A and peptide affinity chromatography. Background Renin is a secreted proteinase whose enzymatic activity is to convert angiotensinogen into angiotensin I in the plasma, initiating a process that results in an elevation of blood pressure and increased sodium retention by the kidney (1). Renin is synthezed in kidney as a procursor, prorenin, which is released into circulation. Both renin and prorenin can bind to (pro)renin receptor and induce angiotensin-independent signaling events leading to activation of MAPKs and up-regulation of TGF-β1 and matrix proteins (2, 3). Defects in renin can cause renal tubular dysgenesis, a severe autosomal recessive disorder of renal tubular development (4, 5). Alternate Names angiotensin-forming enzyme; Angiotensinogenase; FLJ10761; HNFJ2; REN; RENI; Renin; renin precursor, renal Specification REACTIVITY: H SENSITIVITY: Transfected Only MW (kDa): 40-45 SOURCE: Rabbit