CST, 52519T, ASXL1 (D1B6V) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ASXL1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, ASXL1 (D1B6V) Rabbit Monoclonal Antibody (CST #52519) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity ASXL1 (D1B6V) Rabbit Monoclonal Antibody recognizes endogenous levels of total ASXL1 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro1330 of human ASXL1 protein. Background Additional sex combs-like protein 1 (ASXL1) is a polycomb-associated protein that interacts with polycomb repressive complex 2 (PRC2), which contains the histone methyltransferase EZH2 and functions to mono-, di-, and tri-methylate histone H3 on lysine 27. These histone marks are associated with transcriptional repression (1). In addition, ASXL1 interacts with BRCA1-associated protein 1 (BAP1), the catalytic subunit of the polycomb repressive deubiquitinase complex (PR-DUB), which functions to de-ubiquitinate histone H2A at lysine 119 and activate transcription (2). ASXL1 functions as a transcriptional regulator of adipogenesis, acting to repress peroxisome proliferator-activated receptor gamma (PPARG) adipocyte differentiation. ASXL1 also functions as a transcriptional regulator of hematopoiesis acting as an activator of retinoic acid receptor (RAR) mediated transcriptional activation (3,4). ASXL1 is mutated or deleted in 10 to 30 percent of all myeloid malignancies, with loss-of-function mutations associated with poor prognosis in myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML). Hematopoietic-specific deletions of ASXL1 in mice result in progressive, multi-lineage cytopenias and dysplasias, leading to increased number of hematopoietic stem and progenitor cells (5). Alternate Names additional sex combs like 1 (Drosophila); additional sex combs like 1, transcriptional regulator; additional sex combs like transcriptional regulator 1; Additional sex combs-like protein 1; ASXL transcriptional regulator 1; ASXL1; BOPS; KIAA0978; MDS; MGC117280; MGC71111; Polycomb group protein ASXL1; Putative Polycomb group protein ASXL1 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 250 Source/Isotype: Rabbit IgG